These two scans are of ascomycete rDNA that has been
specifically amplified out of: (upper) a sample of naturally
decaying leaf blades of smooth cordgrass from GCE/LTER Station 6 on
Sapelo; (lower) mycelium of a pure culture of Phaeosphaeria spartinicola. The
amplicons were enzyme-cleaved in a standard way such that several
fragments of DNA resulted. The terminal fragment of the digest
contained a fluorescent tag. The digest was separated into its
component fragments by electrophoresis, and the gel was then
scanned for fluorescence. Each species will have its own
particular fluorescent band (shown here as peaks). The P.
spartinicola peak (bottom scan) is clearly visible in the scan
of the leaves from the marsh. Both excellent DNA-tech research and figure
preparation courtesy Alison Buchan (laboratory of Mary Ann Moran).